Recombinant human IL-12/IL-23 (p40) (ELISA Std.)

Bioactive IL-12 is a potent regulator of cell-mediated immune responses and key mediator of Th1 cell development. Bioactive IL-12 is secreted by activated B lymphocytes, dendritic cells and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD and 40 kD subunits. The p40 protein is also a subunit of IL-23 (p60). The disulfide-linked p40 homodimer can bind to IL-12 receptors and antagonize the activities of bioactive IL-12. IL-12 p40 monomer is secreted in vast excess of IL-12 p70 heterodimer; IL-12 p40 monomer has no activity and is antagonistic to IL-12 p70 with only 1/10th the activity of p40 homodimer. Recombinant human IL-12 p40 monomer contains 306 amino acids.\nCytokine detection\nIL-12, IL-23 and IL-35 share common subunits, utilizing combinations of p40, p19 and p35 proteins. Caution must be used when selecting antibodies and assays when specific identification, measurement, as well as activation state discrimination, is required. 1. Active IL-12 consists of two subunits: p40 + p35. 2. p40 can also exist as a monomer (IL-12 p40) or a homodimer (IL-12 p80). 3. Besides contributing to IL-12, p40 is also found in IL-23, a heterodimer of p40 and p19. 4. Similarly, p35 not only contributes to IL-12, but is also found in the heterodimer IL-35 (p35 and EBI3). Note that assays using antibodies specific for the p40 subunit will be unable to discriminate between the active IL-12, monomeric p40, dimeric p40, and IL-23; likewise, assays using p35 detection alone will pick up both IL-12 (heterodimer) and IL-35.

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SPECIFICATIONS

Catalog Number

572109

Size

4 pack

Applications

ELISA

Gene Id

3593

Dilutions

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 4000 pg/ml to 62.5 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store unopened vials at 4C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.

Alternative Names

Interleukin-12 p40, p40 subunit of IL-12 and IL-23, CLMF p40, NKSF p40, TcMF p40, TSF p40

Regulatory Status

RUO