Recombinant mouse IL-12 (p70) (ELISA Std.)

IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Among its biological activities IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-gamma producing Th1 cells. Recombinant murine IL-12 is a 75.0 kD heterodimeric glycoprotein consisting of disulfide-linked 35 kD (p35) and 40 kD (p40) subunits (506 total amino acid residues).

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SPECIFICATIONS

Catalog Number

577009

Size

4 pack

Applications

ELISA

Gene Id

16159,16160

Dilutions

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 4 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store unopened vials at 4C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.

Alternative Names

Interleukin-12 p70, p40 and p35 cystine linked subunits, CLMF, NKSF, TcMF, TSF

Regulatory Status

RUO