The expression plasmid enables for FLP-mediated site specific recombination. The plasmid confers chloramphenicol resistance (708-FLPe) and propagates at 30Degree C in low copy number. flp expression is under control of the themosensitive cI578 promoter and takes place at 37Degree C - 42Degree C.
A temperature upshift from 30Degree C to 37Degree C results in a transient FLPe recombineering activity since the expression plasmids are no longer replicated due to their pSC101-based origin and finally get lost. Compared to the wild type FLP protein FLPe has an improved thermo stability and shows enhanced recombinase activity at 37Degree C
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