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Regulator of G-protein signaling 10, Recombinant, Human, aa1-181, His-tag (RGS10)

Cat no: R1996-21C

Regulator of G-protein signaling 10, Recombinant, Human, aa1-181, His-tag (RGS10)

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 10(RGS10) belongs to this family. All RGS proteins share a conserved 120aa sequence termed the RGS domain. This protein associates specifically with the activated forms of the two related G-protein subunits, G-alphai3 and G-alphaz but fails to interact with the structurally and functionally distinct G-alpha subunits. RGS10 protein is localized in the nucleus. \n\nSource:\nRecombinant corresponding to aa1-181 of human RSG10, fused with His-tag at N-terminus, expressed in E.coli.\n\nAA sequence:\nMGSSHHHHHH SSGLVPRGSH MGSHMFNRAV SRLSRKRPPS DIHDSDGSSS SSHQSLKSTA KWAASLENLL EDPEGVKRFR EFLKKEFSEE NVLFWLACED FKKMQDKTQM QEKAKEIYMT FLSSKASSQV NVEGQSRLNE KILEEPHPLM FQKLQDQIFN LMKYDSYSRF LKSDLFLKHK \nRTEEEEEDLP DAQTAAKRAS RIYNT\n\nMolecular Weight: \n~23.7kD (205aa), confirmed by MALDI-TOF\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R1996-21C

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8, 1mM DTT, 0.1M sodium chloride, 10% glycerol.

Purity

~90% (SDS-PAGE)

References

Cali J J., et al. (1992) J Biol Chem. 267:24023-24027. Simon M I., et al. (1991) Science. 252:802-808.

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