Relative quantification of proteins is a MS-based technique that compares the relative proteins expression levels between different samples, analyzing differential protein expression under varying conditions and revealing the role of proteins in biological processes. Commonly used methods include stable isotope labeling by amino acids in cell culture (SILAC), isobaric tags for relative and absolute quantification (iTRAQ), and tandem mass tags (TMT). They achieve relative quantification by enzymatic digestio
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