Resistin-like Molecule, alpha, Human, Control Peptide (RELMa)

Control Peptide for R1587-06 (antiserum) and R1587-02 (affinity purified)\n\nA 13-aa peptide sequence near the N-terminus of Human RELM-alpha (1) was coupled with KLH.\n\nResistance to insulin characterizes type 2 diabetes, the most common form of diabetes. There is a strong link between type 2 diabetes and obesity, as most patients tend to be obese. Thiozolidinediones (TZDs), a new class of anti-diabetic drugs, enhances target-tissue sensitivity to insulin. A screen of genes down regulated by TZD in adipocyte led to the discovery of a new protein hormone called resistin (for resistance to insulin). Resistin, specifically produced and secreted by adipocyte, is present at elevated levels in the blood of obese animals, and is down regulated by fasting and anti-diabetic drugs. Antibody to resistin stimulated glucose uptake and improved insulin sensitivity in obese mice. Some other protein related to resistin has been called resistin-related molecules (RELM-alpha and beta). Resistin family of proteins was also identified as proteins (FIZZ1-3, for Found in Inflammatory zone) involved in allergy and inflammation.\n\nRELM-alpha/FIZZ1/PMNG1 (Parasite-induced Macrophage Novel Gene 1 (prepeptides: mouse/rat 111 aa; ~77% identity) is 63% related to resistin at the C-terminus (38 aa). The N-terminus of RELM-alpha is less conserved at the N-terminus. RELM-alpha mRNA was most abundant in white adipose tissue. It is also expressed in mammary tissue with significant fat pads. Unlike resistin, RELM-alpha is not expressed in 3T3-L1 adipocyte or in preadipocytes. It is also found in heart, lung and tongue, where resistin is absent. In mice, RELM-alpha/FIZZ1 is found at low levels in a subset of bronchial epithelial cells and in non-neuronal cells adjacent neurovascular bundles in the peribronchial stroma, and in the wall of the large and small bowel. FIZZ1 expression is induced hypertrophic, hyperplastic bronchial epithelium.\n\nApplications: \nSuitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to its low molecular weight. Other applications not tested.\n\nRecommended Dilution:\nELISA: 50-100ng per well.\nAntibody Blocking: 5-10ug per 1ug of R1587-02 (affinity purified antibody) or 1ul R1587-06\n(antiserum).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R1587-05

Size

50ug

Applications

ELISA, WB

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.

Purity

Highly purified

References

(1) Steppan CM et al (2001) Nature 409, 307; Steppan CM et al (2001) PNAS 98, 502; Holcomb IN et al (2000) EMBO J. 19, 4046-4055; Kim K-H et al (2001) J. Biol. Chem. 276, 11252-11256

Additional Info

Sequence has no appreciable homology with mouse/rat RELM-alpha