Resistin-like Molecule, alpha, Recombinant, Mouse (RELMa)

Bronchoalveolar lavage fluid from mice with experimentally induced allergic pulmonary inflammation contains a novel 9.4kD cysteine-rich secreted protein, RELM-alpha (FIZZ1, found in inflammatory zone). RELM-alpha is a secreted protein that has a restricted tissue distribution with highest levels in adipose tissue stroma. Murine RELM-alpha (FIZZ1) is the founding member of a new gene family including two other murine genes expressed, respectively, in intestinal crypt epithelium (RELM-beta) and white adipose tissue (Resistin), and two related human genes. RELMalpha inhibits the differentiation of 3T3-L1 preadipocytes into adipocytes but has no effect on proliferation of 3T3-L1 preadipocytes. RELMalpha is able to form heterooligomers with resistin but not RELMbeta. Since RELMalpha is expressed by adipose tissue and it is a secreted factor, our findings suggest that RELMalpha may be involved in the control of the adipogenesis as well as in the process of muscle differentiation. In the lung, RELM-alpha is induced by hypoxia and was renamed as hypoxia-induced mitogenic factor (HIMF). HIMF strongly activated Akt phosphorylation. The phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 (10 micromol/L) inhibited HIMF-activated Akt phosphorylation. It also inhibited HIMFstimulated RPSM proliferation. Thus, the PI3K/Akt pathway, at least in part, mediates the proliferative effect of HIMF. Further studies showed that HIMF had angiogenic and vasoconstrictive properties. HIMF increased pulmonary arterial pressure and vascular resistence. Further studies suggest that HIMF regulates apoptosis and may participate in lung alveolarization and maturation.\n\nDescription: \nRecombinant Mouse RELM-alpha is manufactured with a signal sequence of phage fd (20 amino acids) and C-terminal fusion of flagTag (10aa). The rmRELM-alpha Flag-Tagged Fusion Protein is a 13.3kD protein containing 91 amino acid residues with 30 additional amino acid residues-signal sequence of phage fd, flagTag.\n\nDescription: \nMKKLLFAIPL VVPFYSHSTM VNTDETIEII VENKVKELLA NPANYPSTVT TLSCTSVKT MNRWASCPAG MTATGCACGF ACGSWEIQSG DTCNCLCLLV DWTTARCCQL SLEDYKDDDD K\n\nSpecificity: \nThe amino acid sequence of the recombinant Mouse RELM-alpha is 100% homologous to the amino acid sequence of the Mouse RELM-alpha.\n\nApplications: \nWestern blotting, ELISA\n\nSolubility: \nAdd 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.\n\nStability: \nStore lyophilized protein at -20 degrees C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degrees C for a limited period of time; it does not show any change after two weeks at 4 degrees C. The lyophilized protein remains stable until the expiry date when stored at -20 degrees C.

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SPECIFICATIONS

Catalog Number

R1587-02B

Size

5ug

Form

Supplied as a lyophilized powder in 5mM Tris, pH 7.5, 25mM sodium chloride.

Purity

(same/more than) 95% (SDS-PAGE); Two-step procedure using size exclusion chromatography before and after refolding.

References

1. FIZZ1/RELMalpha, a novel hypoxia-induced mitogenic factor in lung with vasoconstrictive and angiogenic properties.\n\nCirc Res 2003 May 30;92(10):1065-7