Resistin-like Molecule, beta, Recombinant, Human (RELMb)

RELM-beta (Resistin-Like Molecule-beta) is a member of a recently identified family of secreted proteins containing a conserved cystein-rich C-terminus. The RELM family consists of resistin (also called FIZZ3), RELM-alfa (FIZZ1), RELM-beta (FIZZ2) and RELM-gamma. Only resisistin and RELM-beta were found in humans whereas all four RELM family members were identified in rodents. RELM-beta appears to be produced as a homodimer exclusively by intestinal goblet cells and can be found in high quantities in stool. Remarkably, stool of germ-free mice displaying sterile intestinal tract does not contain RELM-beta until bacterial colonization takes place after pathogen-free mice entered natural environment. Some, but not all, colon carcinoma cell lines secrete RELM-beta into the cell culture supernatant. The physiological function of RELM-beta is not known. High doses of recombinant RELM-beta showed hyperglycemic effects including lowered glucose disposal and increased hepatic glucose production in mice.\n\nDescription: \nRecombinant Human RELM-beta is a His-Tagged Fusion Protein, 11kD protein containing 90 amino acid residues of the human RELM-beta and 12 additional amino acid residues-HisTag (underlined).\n\nAmino Acid Sequence: \nMG STQCSLDS VMDKKIKDVL NSLEYSPSPI SKKLSCASVK SQGRPSSCPA GMAVTGCACG YGCGSWDVQLETTCHCQCSV VDWTTARCCH LTKLRSHHHH HH.\n\nSpecificity: \nThe amino acid sequence of the recombinant human RELM-beta is 100% homologous to the amino acid sequence of the human RELM-beta without signal sequence.\n\nApplications: \nWestern blotting, ELISA\n\nSolubility: \nAdd 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 g/ml. In higher concentrations the solubility of this antigen is limited.\n\nStability: \nStore lyophilized protein at -20 degrees C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degrees C for a limited period of time; it does not show any change after two weeks at 4 degrees C. The lyophilized protein remains stable until the expiry date when stored at -20 degrees C.

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SPECIFICATIONS

Catalog Number

R1587-20B

Size

5ug

Form

Supplied as a lyophilized powder in 0.05M acetate buffer, pH 4.0.

Purity

(same/more than) 95% (SDS-PAGE); Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.

References

1. Human RELMbeta is a mitogenic factor in lung cells and induced in hypoxia.\n\nFEBS Lett 2006 Feb 6;580(3):900-3\n\n2. RELMbeta/FIZZ2 is a goblet cell-specific immune-effector molecule in the gastrointestinal tract.\n\nProc Natl Acad Sci U S A 2004 Sep 14;101(37):13596-600\n\n3. Bacterial colonization leads to the colonic secretion of RELMbeta/FIZZ2, a novel goblet cell-specific protein.\n\nGastroenterology 2003 Nov;125(5):1388-97