Resistin-like Molecule, beta (RELMb)

RELM-beta (resistin-like molecule-beta; FIZZ2) is a 19kD disulfide-linked homodimeric protein expressed in the epithelium of the colon and small bowel. The biological functions of RELM-beta and its molecular targets, are not fully known, but it has been suggested that it plays a regulatory role during inflammation and may also act to establish links among adipose tissue, the intestine and the liver [1]. Interestingly, the molecular structure of RELM-beta is highly homologous to that of the adipose-derived cytokine resistin.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nELISA (Direct and Indirect): 1:5000-1:10,000\nWestern Blot (using ECL): 1:5000-1:10,000. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Incubation time: 1 hour at RT.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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