Resistin, Recombinant, Human (RETN, RETN1, RSTN, Adipose Tissue-specific Secretory Factor, ADSF, C/EBP-epsilon-regulated Myeloid-specific Secreted Cysteine-rich Protein, Cysteine-rich Secreted Protein A12-alpha-like 2, Cysteine-rich Secreted Protein FIZZ3

Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF (Adipose Tissue- Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Human resistin contains 108 amino acids as a prepeptide, and its hydrofobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26. Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppresses the ability of insulin to stimulate glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severly suppressed in obesity and is stimulated by several antidiabetic drugs. Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.\n\n9.9kD protein containing 93aa MSSKTLCSME EAINERIQEV AGSLIFRAIS SIGLECQSVT SRGDLATCPR GFAVTGCTCG SACGSWDVRA ETTCHCQCAG MDWTGARCCR VQP\n\nSpecificity: \nThe amino acid sequence of the recombinant human Resistin is 100% homologous to the amino acid sequence of the human Resistin without signal sequence.\n\nBiological Activity: \nThe biological activity was evidenced by the Resistin ability to prevent insulin-stimulated uptake of deoxy glucose in several cell lines at 10ng/ml concentration.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with ddH2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R1585A

Size

5ug

Form

Supplied as a lyophilized powder in from a concentrated (1mg/ml) solution of 0.03% sodium bicarbonate. Reconstitute with 200ul ddH2O.

Purity

(same/more than) 95% (SDS-PAGE); Two-step procedure using size exclusion chromatography before and after refolding.

References

1. Adiponectin and resistin in the neonatal rat: effects of dexamethasone and hypoxia. Endocrine 2006 Apr;29(2):341-4 2. Resistin serum levels are associated with insulin resistance, disease severity, clinical complications, and prognosis in patients with chronic liver diseases.Am J Gastroenterol 2006 Jun;101(6):1244-523. Serum resistin, adiposity, and insulin resistance in Saudi women: conflicting data or statistical flaws?Ann Saudi Med 2006 Mar-Apr;26(2):1564. Interactions of the hormones leptin, ghrelin, adiponectin, resistin, and PYY3-36 with the reproductive system. Fertil Steril 2006 Jun;85(6):1563-81 5. Leptin and resistin levels and their relationships with glucose metabolism in children with chronic renal insufficiency and undergoing dialysis. Nephrology (Carlton) 2006 Jun;11(3):192-6 6. 17{beta}-Estradiol stimulates resistin gene expression in 3T3-L1 adipocytes via the estrogen receptor, ERK, and C/EBP{alpha} pathways.\nEndocrinology 2006 Jun 1;