Respiratory Syncytial Virus (RSV)

Respiratory syncytial virus (RSV) is the most significant respiratory pathogen of early development and causes about half of all cases of bronchiolitis and a quarter of all cases of pneumonia during the first few months of life. There are multiple types and subtypes of RSV. VP-R151 is a mixture of four antibodies to optimize sensitivity and detection of the various RSV forms. \n\nApplications: \nSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunofluorescence: 1:10\nImmunohistochemistry: 1:200-1:400 for 1 hr at RT Requires antigen retrieval.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nIndirect immunofluorescence: Acetone-fixed HeLa cells infected with RSV-A2. Staining pattern RSV-infected epithelial cell membranes and cytoplasm.\nImmunohistochemistry: Formalin-fixed, paraffin-embedded RSV infected lung.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R1600-13C

Size

1ml

Applications

IF, IHC

Hosts

Mouse

Form

Supplied as lyophilized powder with 15mM sodium azide. Reconstitute with 1ml sterile ddH2O.

P Type

Mab

Purity

Supernatant

Isotype

IgG1+IgG2a

References

1.Wright C, Oliver K C, Fenwick F, et al.. A monoclonal antibody pool for routine immunohistochemical detection of human respiratory syncytial virus anti- gens in formalin-fixed, paraffin-embedded tissue. Journal of Pathology. 182:238-244 (1997). 2.Routledge E C, McQuillan J, Samson A C R, et al.. The development of monoclonal antibodies to respiratory syncytial virus and their use in diagnosis by indirect immunofluorescence. Journal of Medical Virology. 15:305-320 (1985).

Additional Info

Recognizes Respiratory Syncytial Virus. Does not crossreact with tissue culture isolates of influenza virus types A and B, parainfluenza virus types 1, 2, 3 and 4b, adenovirus, herpes simplex virus types 1 and 2, varicella-zoster virus, cytomegalovirus, mumps virus, measles virus, ECHOvirus 19, coxsackie B4 virus, poliovirus types 1, 2 and 3 or negative tissue culture cells used in routine virus isolation.