REST (Repressor Element RE-1 Silencing Transcription Factor, RE1-silencing Transcription Factor, REST Protein, Neural Restrictive Silencer Factor, Neuron Restrictive Silencer Factor, NRSF, Repressor Binding to the X2 Box, XBR)

REST (NRSF/RE1) is a vertebrate silencer protein that potentially regulates a large battery of cell type-specific genes, and therefore may function as a master negative regulator of neurogenesis (1). It is a zinc finger transcription factor that during early embryogenesis is required to repress a subset of neuron-specific genes in non-neural tissues and undifferentiated neural precursors. Changes in REST splicing pattern are suggested to possibly result from altered levels of splicing factors reflecting the formation and/or progression of neuroblastoma tumors (2). REST restricts expression of a large set of genes to neurons by suppressing their expression in non-neural tissues. Its repression involves two distinct repressor proteins, CoREST and mSin3A (3). REST and CoREST together, mediate repression of the type II sodium channel promoter in nonneural cells, and the REST-CoREST complex may mediate long-term repression essential to maintenance of cell identity (4).\n\nApplications: \nSuitable for use in Western Blot, Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:2000\nFlow Cytometry: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

R1623-01C

Size

100ul

Applications

FC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Schoenherr CJ, et al. Science 267(5202):1360-3, 1995. 2. Palm K, et al. Brain Res Mol Brain Res. 72(1):30-9, 1999. 3. Grimes JA, et al. J Biol Chem. 275(13):9461-7, 2000 4. Andr

Additional Info

Recognizes human Rest.