Restriction Enzyme Buffer A, 10X, is specially designed for use in double digestions.
Form (1X Buffer Compostition):
Supplied as a liquid containing 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
Concentration:
Supplied as a 10X concentrated stock. Dilute to 1X or 2X before use.
Storage and Stability:
For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note:
This buffer contains BSA. In order to avoid the risk of bacterial growth, do not store buffer containing BSA at +4 degrees C for periods longer than one week. If buffer is to be stored at 4 degrees C it should be aliquoted into small portions and the main stock should be kept at -20 degrees C
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