Restriction Enzyme Buffer C, 10X, is specially designed for use in double digestions.
Form (1X Buffer Compostition): Supplied as a liquid in 10mM Tris-HCl pH 7.5 10mM magnesium chloride, 50mM sodium chloride, 0.1mg/ml BSA.
Concentration:
Supplied in a 10X concentrated stock.
Dilute to 1X or 2X before use.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note: This buffer contains BSA. In order to avoid the risk of bacterial growth, do not store buffer containing BSA at 4 degrees C for periods longer than one week. If buffer is to be stored at 4 degrees C it should be aliquoted into small portions and the main stock should be kept at -20 degrees C.
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