Ret (c-Ret, Cadherin Family Member 12, CDHF12, ELKS Fusion Gene, HSCR1, Hydroxyaryl Protein Kinase, MEN2A, MEN2B, Multiple Endocrine Neoplasia and Medullary Thyroid Carcinoma 1, MTC1, Oncogene RET, Proto-oncogene Tyrosine Protein Kinase Receptor Ret, PTC,

The ret proto-oncogene encodes a cell surface glycoprotein belonging to a member of the receptor tyrosine kinase family and is located on chromosome 10q11.2. Three main 3' splice isoforms have been characterized from papillary thyroid carcinomas, themselves originating from thyroid epithelial cells. Ret expression is observed in several regions of the central nervous system; in the developing cranial nerve ganglia and a subset of cells within dorsal root ganglia, in motor neurons in the spinal cord and hindbrain, in neuroretina and the growing tips of the renal collecting ducts in developing kidney.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Formalin/Paraffin): 1:15-1:30 for 90 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nEnteric ganglion cells. Small intestine.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

R1640

Size

500ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, 0.09% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG1

References

1. Nosrat, C.A., et al., Cellular and developmental patterns of expression of Ret and glial cell line-derived neurotrophic factor receptor alpha mRNAs. Brain Res. 115: 410-422 (1997). 2. Williams, G.H., et al., Analysis of the ret proto-oncogene in sporadic parathyroid adenomas. Journal of Pathology. 180: 138-141 (1996). 3. Nakamura, T., et al., Expression of the Ret proto-oncogene product in human normal and neoplastic tissues of neural crest origin. Journal of Pathology. 172: 255-260 (1994).

Additional Info

Recognizes human Ret oncoprotein.