Retinoblastom Antibody Sampler Kit, BioAssay(TM) (Rb)

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.\n\nApplications: \nSuitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R1660-01D

Size

4x40ul

Applications

ELISA, FC, ICC, IHC, IP, WB

Reactivities

Hum, Mouse, Rat, Bov, NHP, ZF/Fish

Form

Supplied as a liquid in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol.

Purity

Purified by Protein A and peptide affinity chromatography.

References

1. Sherr, C.J. (1996) Science 274, 1672-1677. 2. Nevins, J.R. et al. (1992) Science 258, 424-429. 3. Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-790. 4. Hu, Q.J. et al. (1990) EMBO J. 9, 1147-1155. 5. Knudsen, E.S. and Wang, J.Y. (1997) Mol. Cell. Biol. 17, 5771-5783. 6. Lundberg, A.S. and Weinberg, R.A. (1998) Mol. Cell. Biol. 18, 753-761. 7. Connell-Crowley, L. et al. (1997) Mol. Cell. Biol. 8, 287-301. 8. Kitagawa, M. et al. (1996) EMBO J. 15, 7060-7069. 9. Geng, Y. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 194-199.

Additional Info

Detect endogenous levels of Rb protein only when phosphorylated at Ser780, Ser795, and Ser807/811, respectively.