Retinoblastoma Binding Protein 7 (RbBP7, RbBP-7, Chromatin Assembly Factor 1 Subunit C, G1/S Transition Control Protein Binding Protein RbAp46, Histone Acetyltransferase Type B Subunit 2, Histone Binding Protein RbBP7, MGC138867, MGC138868, MGC53418, MSI1

Retinoblastoma protein (Rb) associated protein 46 (RbAp 46) is a nuclear protein that has been shown to interact directly with retinoblastoma protein and is a component of the mSin3 corepressor complex as are the histone deacetylase proteins (HDACs). RbAp 46 shares about 75% sequence identity with the closely related RbAp 48, and appears to be functionally related to the yeast MSI 1 protein in that overexpression of RbAp 46 suppresses heat shock sensitivity in a RAS pathway dependent manner. RbAp 46 has also been demonstrated to be up-regulated by the Wilms? tumor suppressor gene product, WT1. Additionally, cells transfected with RbAp46 have shown strong inhibition of growth.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5ug/ml\nImmufluorescence: 1ug/ml\nImmunocytochemistry: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R1665-36

Size

100ug

Applications

ICC, IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Guan LS et al. Induction of Rb-associated protein (RbAp46) by Wilms' tumor suppressor WT1 mediates growth inhibition. J Biol Chem 273:27047-50 (1998). <PUBMED:9765217>Brehm A et al. Retinoblastoma protein recruits histone deacetylase to repress transcription. Nature 391:597-601 (1998). <PUBMED:9468139>Qian YW & Lee EY Dual retinoblastoma-binding proteins with properties related to a negative regulator of ras in yeast. J Biol Chem 270:25507-13 (1995). <PUBMED:7503932>

Additional Info

Recognizes human RbBP7. Species sequence homology: mouse and rat (100%), Xenopus laevis (92%), chicken (84%). Cellular Localization: Nuclear.