Retinoblastoma (Rb) (BSA & Azide Free)

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia. \n\nMW of Antigen: 105kD \n\nEpitope: aa703-722 \n\nPositive Control: Ls174T, HT29, HUVEC cells. Normal colon and carcinoma. Breast carcinoma. \n\nCellular Localization: Nuclear \n\nApplications and Recommended Dilutions: \nFlow Cytometry \nImmunofluorescence1 \nImmunoprecipitation (Native and denatured) (Use Protein G) (Ab 2ug/mg protein lysate) \nWestern Blotting (Ab 1-2ug/ml for 2hrs at RT) \nImmunohistochemistry (Formalin/paraffin) (Ab 1-2ug/ml for 30 min at RT) * (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0 , for 10-20 min followed by cooling at RT for 20 min.) [EDTA is better than citrate for epitope unmasking] The optimal dilution for a specific application should be determined by the investigator.\n\nStorage and Stability:\n May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degrees C or colder. Aliquots are stable for at least 12 months at-20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R1660-18X

Size

100ul

Applications

FC, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See R1660-18.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Bartek J, et. al. Oncogene 7:101

Additional Info

Specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells.1 It reacts with hyperphosphorylated and un (under) phosphorylated form of Rb protein. It shows no crossreaction with p107 and p130. Species Crossreactivity: human. Does not react with mouse and rat.