Retinoblastoma (Rb, Retinoblastoma 1, Retinoblastoma 1 (Including Osteosarcoma), Rb1, Rb1 Protein, Rb-1, Including Osteosarcoma, OSRC, p105 RB, p105-RB, PP105, PP110, pRb, Retinoblastoma-associated Protein, Retinoblastoma-related Osteosarcoma, Retinoblast

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of ts function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.\n\nApplications: \nSuitable for use in RT, Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry (paraffin-embedded sections). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: Nuclear\n\nPositive Control: Ls174T, HT29, HUVEC cells. Normal colon and colonic / breast carcinoma tissue sections.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R1660-07D

Size

500ul

Applications

FC, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

0.2% BSA, 10mM PBS. pH 7.4

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Aagaard L; Lukas J; Bartkova J; Kjerulff AA; Strauss M; Bartek J. Aberrations of p16Ink4 and retinoblastoma tumour-suppressor genes occur in distinct sub-sets of human cancer cell lines. International Journal of Cancer, 1995, 61(1):115-20.\n2. Bartkova J; Lukas J; Muller H; Strauss M; Gusterson B; Bartek J. Abnormal patterns of D-type cyclin expression and G1 regulation in human head and neck cancer. Cancer Research, 1995, 55:949-56.\n3. Bartkova J; Lukas J; Muller H; Lutzhoft D; Strauss M; Bartek J. Cyclin D1 protein expression and function in human breast cancer. International Journal of Cancer, 1994, 57(3):353-61.\n4. Bartkova J; Lukas J; Strauss M; Bartek J. The PRAD-1/cyclin D1 oncogene product accumulates aberrantly in a subset of colorectal carcinomas. International Journal of Cancer, 1994, 58(4):568-73.\n5. Hickman ES; Picksley SM; Vousden KH. Cells expressing HPV16E7 continue cell cycle progression following DNA damage induced p53 activation. Oncogene, 1994, 9:2177-81.\n6. Lukas J; Jadayel D; Bartkova J; Nacheva E; Dyer MJ; Strauss M; Bartek J. BCL-1/cyclin D1 oncoprotein oscillates and subverts the G1 phase control in B-cell neoplasms carrying the t(11;14)\ntranslocation. Oncogene, 1994, 9(8):2159-67. 7. Lukas J; Muller H; Bartkova J; Spitkovsky D; Kjerulff AA; Jansen-Durr P; Strauss M; Bartek J. DNA tumor virus oncoproteins and retinoblastoma gene mutations share the ability to relieve the cell's requirement for cyclin D1 function in G1. Journal of Cell Biology, 1994, 125(3):625-38.\n8. Bartek J, Vojtesek B, Grand RJA, Gallimore PH, and Lane DP. Cellular localization and T antigen binding of the retinoblastoma protein. Oncogene 7:101-108, 1992.

Additional Info

Specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. Reacts with hyperphosphorylated and un (under) phosphorylated forms of Rb protein. It shows no crossreaction with p107 and p130. Species Crossreactivity: Crossreacts with Human. Does not crossreact with Mouse or Rat.