Retinoblastoma (Rb, Retinoblastoma 1, Retinoblastoma 1 (Including Osteosarcoma), Rb1, Rb1 Protein, Rb-1, Including Osteosarcoma, OSRC, p105 RB, p105-RB, PP105, PP110, pRb, Retinoblastoma-associated Protein, Retinoblastoma-related Osteosarcoma, Retinoblast

Rb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F1, PU1, ATF2, UBF, Elf1 and cAbl. This ability of Rb to alter transcription is regulated by phosphorylation catalyzed by the cyclin dependent protein kinases (cdks). Rb is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus 16E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the Rb gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: Nuclear\n\nPositive Control: Jurkat cells in high growth phase.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R1660-07M

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

0.05% Sodium Azide; PBS, 1.0mg/ml BSA (IgG, protease free). pH 7.4

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

This product has been used in:Law BK et al. Rapamycin potentiates transforming growth factor beta-induced growth arrest in nontransformed, oncogene-transformed, and human cancer cells. Mol Cell Biol 22:8184-98 (2002). <PUBMED:12417722>Glassford J et al. Vav is required for cyclin D2 induction and proliferation of mouse B lymphocytes activated via the antigen Receptor. J Biol Chem 276:41040-8 (2001). <PUBMED:11546804> Rubin E et al. Site-specific and temporally-regulated retinoblastoma protein dephosphorylation by protein phosphatase type 1. Oncogene 20:3776-85 (2001). <PUBMED:11439341>Brantley MA & Harbour JW The molecular biology of retinoblastoma. Ocul Immunol Inflamm 9:1-8 (2001). <PUBMED:11262663>

Additional Info

Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse (100% identity with immunogen), Rat (100% identity with immunogen) due to sequence homology. Not yet tested in other species.