Retinoic Acid Receptor beta, CT (Retinoic Acid Receptor beta Polypeptide, RAR beta, RAR-beta, RARb, Hepatitis B Virus Activated Protein, Hepatitis B Virus-activated, HBV Activated Protein, HBV-activated Protein, HAP, Nuclear Receptor Subfamily 1 Group B M

Retinoic acid receptor beta (RAR beta) is a 55kD transcriptional factor which is activated by retinoic acid. Once activated RAR beta heterodimerizes with retinoid X receptors (RXRs) and translocates to the nucleus, where it stimulates the transcription of growth-inhibitory, differentiation-inducing genes. In this way RAR beta mediates cellular signalling in embryonic morphogenesis and organogenesis. The RAR beta exists as three isoforms, which are localised to the cytoplasm or subnuclear compartments. Down-regulation of the RAR-b2 isoform occurs early in tumorigenesis, and is a tumor suppressor gene.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 5-15ug/ml\nRequires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections. 0.01 M sodium citrate buffer, pH 6.0 is recommended.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nIntermediate trophoblasts\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R1691-05M

Size

50ug

Applications

IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH7.7, 0.09% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Swift, ME. et al. (2006) Truncated RAR beta isoform enhances proliferation and retinoid resistance. J Cell Physiol. 209: 718-725.2. Desai, T.J. et al. (2006) Distinct roles for retinoic acid receptors alpha and beta in early lung morphogenesis. Dev. Biol. 291: 12-24.3. Grunt, T.W. et al. (2005) An EGF receptor inhibitor induces RAR-b expression in breast and ovarian cancer cells. Biochemical and Biophysical Research Communications. 329: 1253-1259.

Additional Info

Recognizes an epitope within the C-terminal of human retinoic acid receptor beta. Does not crosreact with rat.