Retinoic Acid Receptor beta (RAR beta, RARb))

Retinoids can reverse various premalignant lesions as well as prevent some second primary tumors. The effects of retinoids are regulated by retinoic acid receptors (RAR) and retinoid X receptors, which act as ligand-activated transcription factors. Ligand-dependent transcriptional activation of RARs is a multistep process that ends with the formation of a multimeric co-activator complex on regulated promoters (1,2). Loss of RAR-beta expression and the accumulation of p53 and Ki67 proteins may help in the early identification of esophageal cancer in high-risk populations (3).\n\nApplications:\nSuitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Recognizes a ~48kD band using rat hippocampal homogenates. Tested in TBST (20mM Tris HCl, pH 7.6, 137mM NaCl, 1% Tween 20, 5% nonfat milk, 0.05% azide.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R1691-05

Size

50ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Rat

Form

Supplied as a liquid in 10 mM Hepes, pH7.5, 150 mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Klaassen, I., et al. Expression of retinoic acid receptor gamma correlates with retinoic acid sensitivity and metabolism in head and neck squamous cell carcinoma cell lines. Int. J. Cancer. 92(5):661-665, 2001. 2. Lefebvre, B., et al. Phosphorylation of Histone H3 is functionally linked to retinoic acid receptor {beta} promoter activation. EMBO. 3(4): 335-340, 2002. 3. Xu, M., et al. The abnormal expression of retinoic acid receptor-beta, p53 and Ki67 protein in normal, premalignant, and malignant esophageal tissues. World J. Gastroenterol. 8(2): 200-202, 2002.

Additional Info

Specific for Retinoic acid receptor, beta isotype. Species Crossreactivity: Human and rat RAR beta.