Retinol Binding Protein 1 (RBP1, CRBP, Cellular Retinol Binding Protein)

Domain Information: \n\nGene Ontology Terms: lipid binding; retinol binding; transport; transporter activity; vitamin A metabolism\n\nApplication(s):\nPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 16kD band observed in Murine NIH 3T3 lysates (calculated MW of 15.9kD according to NP_002890.1). Recommended concentration: 0.1-0.3ug/ml.\n\n0.1ug/ml staining of NIH 3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See R1701-18-P. Antibody

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SPECIFICATIONS

Catalog Number

R1701-18

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

Farias EF, Ong DE, Ghyselinck NB, Nakajo S, Kuppumbatti YS, Mira y Lopez R. , Cellular retinol-binding protein I, a regulator of breast epithelial retinoic acid receptor activity, cell differentiation, and tumorigenicity., J Natl Cancer Inst. 2005 Jan 5;97(1):21-9.

Additional Info

Recognizes Human, Mouse RBP1