The RBP Immunoassay Kit is designed to measure RBP levels in serum and EDTA and heparin plasmas. This kit uses a native human RBP molecule as a standard, the concentration of which has been established via amino acid analysis. Standards or diluted samples are pipetted into a microtiter plate coated with a goat antibody to rabbit IgG. A RBP-HRP conjugate is added and the binding reaction initiated by addition of a RBP-specific rabbit polyclonal antibody. After a 60-minute incubation, the plate is washed and TMB substrate solution added to each well. After 15 minutes the intensity of the generated signal is read at 450 nm in a microtiter plate reader.
Retinol binding protein (RBP) is a 21 kDa highly conserved, single-chain glycoprotein that has a hydrophobic pocket which binds retinol (vitamin A) in a 1:1 stoichiometry, which serves to not only solubilize retinol but also protect it from oxidation. RBP has been shown to be a useful surrogate marker for retinol status in populations. The World Health Organization has estimated that 250 million children have moderate to severe Vitamin A deficiency due to lack of adequate nutrition. Measurement of RBP levels has also been useful in detection and characterization of diseases including chronic renal insufficiencies, liver disease and cancers.
Human serum and EDTA and heparin plasmas have been validated with this assay.
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