RGS19IP1 (RGS19IP1, NIP, GIPC, IIP-1, TIP-2, SEMCAP, C19orf3, Hs.6454, GLUT1CBP, SYNECTIIN, regulator of G-protein signalling 19 interacting protein 1, synectin, GLUT1 C-terminal binding protein, chromosome 19 open reading frame 3, IGF-1 receptor interact

Alternate Name: RGS19IP1, NIP, GIPC, IIP-1, TIP-2, SEMCAP, C19orf3, Hs.6454, GLUT1CBP, SYNECTIIN, regulator of G-protein signalling 19 interacting protein 1, synectin, GLUT1 C-terminal binding protein, chromosome 19 open reading frame 3, IGF-1 receptor interacting protein 1\n\nDomain Information: Domain present in PSD-95, Dlg, and ZO-1/2\n\nGene Ontology Terms: protein binding; G-protein coupled receptor protein signaling pathway; soluble fraction; membrane fraction\n\nApplication(s):\nPeptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 40kD band observed in Hela and Human Kidney lysates (predicted MW of 38kD according to NP_005707.1). Recommended for use at 1-3ug/ml.\n\nStaining (1ug/ml) of Hela lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.\n\nImmunohistochemistry: In paraffin embedded Human Kidney, shows membrane and/or cytoplasmic staining of epithelial cells of renal tubules and some rare cells of the glomeruli. Recommended concentration, 10ug/ml.\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See R1996-39C-P. Antibody

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SPECIFICATIONS

Catalog Number

R1996-39C

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

De Vries L, Lou X, Zhao G, Zheng B, Farquhar MG. , GIPC, a PDZ domain containing protein, interacts specifically with the C terminus of RGS-GAIP., Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12340-5.

Additional Info

This antibody is expected to recognize both isoform 1 (NP_005707.1, NP_974197.1 and NP_974199.1) and isoform 2 (NP_974196.1, NP_974198.1 and NP_974223.1). Species Crossreactivity: human, mouse, rat.