Rheb1

Rheb proteins represent a novel and unique family of the Ras superfamily GTP-binding proteins that is conserved from yeast to human. Biochemical studies establish that they bind and hydrolyze GTP. Molecular modeling studies reveal a few structural differences between Rheb and Ras, which may suggest that residues involved in biochemical activities differ between the two G-proteins. The function of Rheb has been studied in a number of organisms that point to the involvement of Rheb in cell growth and cell cycle progression. In addition, studies in fungi suggest that Rheb is involved in arginine uptake. Further studies in Drosophila and mammalian cells have shown that the effects of Rheb on growth and cell cycle progression are mediated by the effect on the insulin/TOR/S6K signaling pathway. These studies have also shown that a complex consisting of the tuberous sclerosis gene products, Tsc1/Tsc2, serves as a GTPase activating protein (GAP) for Rheb, implying Rheb's role in this genetic disorder. Finally, Rheb proteins have been shown to be farnesylated and small molecule inhibitors of protein farnesyltransferase can block the ability of Rheb to activate the TOR/S6K signaling.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nQuality Control Testing\nImmunoblot Analysis: A 1:500 (1ug/mL) dilution of this lot detected Rheb1 in rat brain microsomal lysate. Immunoprecipitation: Immunoblot Analysis Lysates from rat brain microsomal was resolved by electrophoresis, transferred to PVDF (Immobilon-P), and probed with anti-Rheb1 for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates Rheb1.

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SPECIFICATIONS

Catalog Number

R1996-75

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 0.02 M PBS, 0.25 M sodium chloride(pH 7.6) containing 0.1% sodium azide.

P Type

Pab

Purity

Purified by Protein A.

Isotype

IgG

References

1. Aspuria, Paul-Joseph and Tamanoi, Fuyuhiko (2004). The Rheb family of GTP-binding proteins. Cell Signal 16: 1105-12.\n2. Saucedo, Leslie J, et al (2003). Rheb promotes cell growth as a component of the insulin/TOR signalling network. Nat Cell Biol 5: 566-71.\n3. Tabancay, Angel P, et al (2003). Identification of dominant negative mutants of Rheb GTPase and their use to implicate the involvement of human Rheb in the activation of p70S6K. J Biol Chem 278: 39921-30.

Additional Info

Recognizes Rheb1, not Rheb2.