Rheumatoid Factor IgM, Human, BioAssay(TM) ELISA Kit

Intended Use:\nFor quantitative determination of Rheumatoid Factor IgM in human serum.\n\nTest Principle:\nRheumatoid Factor IgM (RF IgM) ELISA kit is based on binding of RF IgM from serum samples to human gamma globulin immobilized on microtiter wells. After a washing step, anti-human IgM-HRP conjugate is added. After another washing step, to remove all the unbound enzyme conjugate, chromogenic substrate is added and color developed. The enzymatic reaction (color) is directly proportional to the amount of RF IgM present in the sample. Adding stopping solution terminates the reaction. Absorbance is then measured on a microtiter well ELISA reader at 450nm and the concentration of RF IgM in samples is calculated on the basis of the absorbance of the negative, positive, and, calibrator controls.\n\nKit Components:\nR1997-06A: Microwell plate, 1x96 wells\nR1997-06B: Negative Control (<25IU/ml)(100X), 1x300ul\nR1997-06C: Positive Control (40-90IU/ml) (100X), 1x300ul\nR1997-06D: Calibrator (100IU/ml) (100X), 1x300ul\nR1997-06E: Sample Diluent, 1x50ml\nR1997-06F: (HRP) Conjugate, 1x11ml\nR1997-06G: Wash Buffer (100X), 1x50ml\nR1997-06H: TMB Substrate, 1x11ml\nR1997-06J: Stop solution, 1x10ml\n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R1997-06

Size

96Tests

Applications

ELISA

References

1. Johnson PM et al (1976) Clin. Immunol. 6, 414. 2. Ziff M et al (1985) in Autoimmune Diseases, 59-79. 3. Bartfield H et al (1969) Ann. NY Acad. Sci. 168, 30. 4. Karah J et al (1980) J Immunol. Meth. 32, 115. 5. Moors TL et al (1978) Arth Rheumat. 21, 935. 6. Seymour P et al (1980) Am. Soc. Clin. Pathol. 74, 776. 7. Puente A et al (1988) Arth. Rheumat. 31, 1230.