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RhoD, Recombinant, Human, aa18-207, His-tag (ARHD, RHOHP1, RHOM)

Cat no: R1998-10Z

RhoD, Recombinant, Human, aa18-207, His-tag (ARHD, RHOHP1, RHOM)

Rho-related GTP-binding protein, also known as RhoD, belongs to the small GTPase superfamily. The small GTPase Rho D contributes to rearrangement of the actin cytoskeleton and cell surface and also governs endosome motility and distribution. \n\nSource: \nRecombinant corresponding to aa18-207 from human RhoD, fused to His-tag at N-terminus expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MVKVVLVGDG GCGKTSLLMV FADGAFPESY TPTVFERYMV NLQVKGKPVH LHIWDTAGQD DYDRLRPLFY PDASVLLLCF DVTSPNSFDN IFNRWYPEVN HFCKKVPIIV VGCKTDLRKD KSLVNKLRRN GLEPVTYHRG QEMARSVGAV AYLECSARLH \nDNVHAVFQEA AEVALSSRGR NFWRRITQGF C\n\nMolecular Weight: \n~23.8kD (211aa), confirmed by MALDI-TOF\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R1998-10Z

Size

10ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 5mM DTT, 200mM sodium chloride, 2mM EDTA, 0.1mM PMSF, 50% glycerol.

Purity

~90% (SDS-PAGE)

References

1. Regnauld, K., et al. (2002) Oncogene. 21:4020-4031. 2. Tsubakimoto., et al. (1999) Oncogene. 18:2431-2440.

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