Rhodopsin Kinase 1a,b (G Protein-Associated Rhodopsin Kinase 1a/b, GRK1a/b)

The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein-coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events. \n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence 1:1000; Immunofluorescence staining of GRK1a/b in bovine retina results in intense staining primarily in cone outer segments, but also in rod outer segments. Weak staining is observed in somata and synaptic terminals of cones and inner segments of rods. \nWestern Blot 1:1000; Detects an ~60kD protein representing GRK 1a/b. Detects both in vitro expressed splice variants by Western blot but was not able to detect significant amounts of GRK1b from human retina. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R1998-19A

Size

100ug

Applications

IF, IP, WB

Hosts

Mouse

Reactivities

Bov

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Mab

Purity

IgG fraction.

Isotype

IgG

References

Bensaoula T et al. Histopathologic and immunocytochemical analysis of the retina and ocular tissues in Batten disease. Ophthalmology 107 :1746-53 (2000). PubMed: 10964839 \n\nZhao X et al. A novel form of rhodopsin kinase from chicken retina and pineal gland. FEBS Lett 454 :115-21 (1999). PubMed: 10413107.

Additional Info

Detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues. Does not crossreact with other G protein-coupled kinases. Epitope. Recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b.