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RhoGDI (Rho Guanine Dissociation Inhibitor)

Cat no: R1998-20

RhoGDI (Rho Guanine Dissociation Inhibitor)

GDP-Dissociation Inhibitors (GDIs) modulate GTPase activity by binding to small G-Proteins, and regulating GDP/GTP exchange. Members of this family of proteins include Rho-GDI (GDI-1), D4-GDI (GDI-2) and Rhoto Rho and Rab, and D4-GDI, which was recently described as -GDI-3 (GDI-3). Rho-GDI, which can bind being a substrate for Caspase 3 (CPP32/YAMA/Apopain) during Fas-induced apoptosis, are both localized in the cytosol. In contrast, GDI-3 may associate with the membrane and cytoskeleton, primarily binding to RhoB and RhoG. Rho-GDI and D4-GDI are highly homologous, differing primarily in the first 25 amino acids of the N-Terminus.\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 0.5-1ug/ml detects Rho-GDI in RIPA lysates from human A431, HL-60, HeLa, Hs294T, Jurkat and murine 3T3/NIH cells. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with R198-20 (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.\nImmunoprecipitation: 4ug immunoprecipitates Rho-GDI from 500ug of Jurkat RIPA lysate.\nOptimal dilutions to be determined by the researcher.\n\nIncluded Positive Antigen Control: \nF4209: Fibroblast Cell, 3T3/A31 lysate. Add 2.5ul 2-mercaptoethanol/100ul of lysate. Boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for Western Blot analysis.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R1998-20

Size

200ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Bov

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1.

Additional Info

Recognizes human Rho-GDI. Crossreacts with D4-GDI. Species Crossreactivity: bovine and mouse.

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