Ribonuclease P Protein Subunit p20 (RNaseP Protein p20, RPP20, 0610037N12Rik, hPOP7, Processing of Precursor 7 Ribonuclease P/MRP Subunit (S. cerevisiae), POP7, Ribonucleases P/MRP Protein Subunit POP7 Homolog, RPP2)

The nuclei of eukaryotic cells contain several classes of small RNA-protein complexes SnRNPs (small nuclear ribonucleoproteins) are involved in splicing of pre-mRNAs (the excision of introns) in the nucleoplasm. RPP20 is part of such a complex. SnoRNPs (small nucleolar ribonucleoproteins) are involved in the maturation of pre-ribosomal RNA in the nucleoli.\nPre-rRNA processing and modification require both the snoRNA and the protein constituents (such as Rpp20) of these complexes. Generally, snoRNPs contain a number of common\nproteins, which are shared with other snoRNPs of the same family, next to several particle-specific proteins. On average, snoRNPs contain about 6-10 protein subunits.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R2027-95

Size

1ml

Applications

ELISA

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in 10mM ammonium bicarbonate buffer. Reconstitute with ddH2O to 0.1-1ml.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Welting et al. Autoantigenicity of nucleolar complexes. Autoimmun Rev. 2. Van Eenennaam et al. Architecture and function of the human endonucleases RNase P and RNase MRP. IUBMB Life. 2000 Apr;49(4):265-72. 3. Welting et al. Mutual interactions between subunits of the human RNase MRP ribonucleoprotein complex. Nucleic Acids Res. 2004 Apr 19;32(7):2138-46. Print 2004.

Additional Info

Recognizes recombinant GST-human RPP20.

Alternative Names

EC=3.1.26.5