Ribosomal P, Human, IgG, IgA, and IgM BioAssay(TM) ELISA Kit

Ribosomal P, Human, IgG, IgA, and IgM BioAssay(TM) ELISA Kit is intended for the detection and semi-quantitative determination of IgG, A, M antibodies to Ribosomal P in human serum. The assay is to be used to detect antibodies in a single serum specimen. The results of the assay are to be used as an aid in the diagnosis of Systemic Lupus Erythematosus (SLE). Systemic autoimmune disease is characterized by the presence of circulating autoantibody directed to a wide variety of cellular antigens. Systemic lupus erythematosus (SLE) commonly referred to as Lupus is the best known of these diseases. Other possible connective tissue diseases include mixed connective tissue disease (MCTD), Sjogren syndrome, scleroderma, and polymyositis/dermatomyositis. The majority can be diagnosed by clinical presentation and their antibody profiles to the various antigens involved, which include dsDNA, Sm, RNP, Ro, La, Scl-70, Jo-1 and Histones. Therefore, immunoassays for autoantibody are useful for diagnostic and prognostic evaluations of autoimmune disease. The Ribosomal P test is an Enzyme-Linked Immunosorbent Assay to detect IgG, IgA, and IgM antibodies to Ribosomal P antigens. Purified Ribosomal P antigens are attached to a solid phase microassay well. Enzyme-Linked Immunosorbent Assays (ELISA) relies on the ability of biological materials (i.e., antigens) to adsorb to plastic surfaces such as polystyrene (solid phase). When antigens bound to the solid phase are brought into contact with a patient\\'s serum, antigen specific antibody, if present, will bind to the antigen on the solid phase forming antigen- antibody complexes. Excess antibody is removed by washing. This is followed by the addition of goat anti-human IgG, A, M conjugated with horserumdish peroxidase which then binds to the antibody-antigen complexes. The excess conjugate is removed by washing, followed by the addition of Chromogen/Substrate, tetramethylbenzidine (TMB). If specific antibody to the antigen is present in the patient\\'s serum, a blue color develops. When the enzymatic reaction is stopped with 1N H2SO4, the contents of the wells turn yellow. The color, which is indicative of the concentration of antibody in the serum, can be read on a suitable spectrophotometer or ELISA microwell plate reader.\n\nPrinciple: ELISA-Peroxidase conjugated\nSpecificity: ~ 99%\nSensitivity: ~100%\n\nKit Components: \nEach kit contains the following components in sufficient quantities to perform the number of tests indicated. All reactive reagents contain 0.1% sodium azide as a preservative \n\n1. Microtiter Plate\n2. Conjugate\n3. Calibrator\n4. Positive Control\n5. Negative Control\n6. TMB\n7. Wash Buffer, 10X Diluted wash buffer to 1X\n8. Sample Diluent\n9. Stop Solution \n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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