Ribosomal P

The Ribosomal P antigen consists of three proteins of the 60S ribosomal subunit. These three proteins are designated PO (38kD), P1(19kD), and P2(17kD). Studies have suggested that one P1 homodimer and one P2 homodimer are attached to PO by the NH2-termini. P1 and P2 are believed to be the eukaryotic equivalent of the E. coli ribosomal protein L12 and have been shown to contain sequences that are highly conserved among eukaryotes. The major immunoreactive epitope of these ribosomal antigens has been mapped and is localized to the carboxy terminus. PO, P1, and P2 have an identical 17 amino acid carboxy terminus. The Ribosomal P antigens have GTPase activity and have been shown to be phosphorylated.\n\nApplications: \nSuitable for use in ELISA and Immunodiffusion. Other applications not tested.\n\nRecommended Dilutions:\nELISA: >1:100\nOptimal dilutions to be determined by the researcher.\n\nTiter:\nImmunodiffusion: 1:4\n\nELISA Results: \nInterpretation of Results: <16EU/ml: Negative; 16-20EU/ml: Equivocal; Positive; >20EU/ml:\nSSA: 4EU/ml\nSSB, SCL: 2EU/ml\nSm: 1EU/ml \nSm/RNP: 19EU/ml \nJo-1: 3EU/ml\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

R2031-25

Size

2ml

Applications

ELISA

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.2, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.

P Type

Pab

Purity

Immunoglobulin fraction obtained from precipitation of delipidized and defibrinated single donor human plasma.

Isotype

IgG

Additional Info

Recognizes human Ribosomal P antigen. Shows only the anti-Ribosomal P precipitin band when tested by double immunodiffusion against rabbit thymus extract. Does not recognize SSA, SSB, Sm, Sm/RNP and Jo-1 antigen when used at 1:100 dilution in an ELISA system designed to detect all IgG subclasses.