Ribosomal Protein S19 (RPS19, 40S Ribosomal Protein S19, DBA, HGNC:10402)

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19E family of ribosomal proteins. It is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia (DBA), a constitutional erythroblastopenia characterized by absent or decreased erythroid precursors, in a subset of patients. This suggests a possible extra-ribosomal function for this gene in erythropoietic differentiation and proliferation, in addition to its ribosomal function. Higher expression levels of this gene in some primary colon carcinomas compared to matched normal colon tissues has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nPeptide ELISA Titer: 1:64,000\nWestern Blot: 0.3-1ug/ml ~15kD band observed in Human lysates of cell lines Daudi and K562 (calculated MW of 16.1kD according to NP_001013.1). In transfected HEK293 transiently expressing RPS19 a band of approx. 18kD is observed. This band is not observed in the non-transfected HEK293. \nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R2031-39U

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1. Leger-Silvestre I, Caffrey JM, Dawaliby R, Alvarez-Arias DA, Gas N, Bertolone SJ, Gleizes PE, Ellis SR. \nSpecific role for yeast homologs of the diamond blackfan anemia associated Rps19 protein in ribosome synthesis.J Biol Chem. 2005 Sep 12; [Epub ahead of print]

Additional Info

Recognizes human Ribosomal Protein S19 . Species sequence homology: mouse, rat, canine, bovine.

Alternative Names

40S ribosomal protein S19, DBA, HGNC:10402, ribosomal protein S19, RPS19