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Ribosomal Protein S6 Kinase, p70 (p70S6 Kinase, EC 2.7.11.1, p70(S6K)-alpha, p70S6K, PS6K, RPS6KB1, S6Kalpha, STK14A)

Cat no: R2031-38C1

Ribosomal Protein S6 Kinase, p70 (p70S6 Kinase, EC 2.7.11.1, p70(S6K)-alpha, p70S6K, PS6K, RPS6KB1, S6Kalpha, STK14A)

The protein p70 S6 kinase is critical for cell cycle progression and cell survival. In response to mitogen stimulation, p70 S6 kinase activation up- regulates ribosomal biosynthesis and enhances the translational capacity. The p70S6K phosphorylates the S6 protein of the 40S subunit of the ribosome. This kinase was first characterized as an insulin/mitogen- activated protein kinase, whose major known substrate is the 40 S ribosomal subunit protein S6. The p70 S6 kinase is activated by diverse stimuli through a multi-site phosphorylation such as Thr-252 and Ser- 434. In Alzheimer's disease (AD), p70 S6 kinase activation is associated with PHF-tau (hyperphosphorylated tau) accumulation. In non-neuronal cells, p70 S6 kinase have been shown to regulate actin polymerization.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R2031-38C1

Size

50ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. An, W. et al. Am J Pathol 163, 591 (2003). 2. Raymond, C. et al. Neuroscience 109, 531(2002). 3. Avruch, J. et al. Mol Cell Biochem 182, 31(1998). 4. Weng, Q. et al. J Biol Chem 273, 16621 (1998). 5. Price, D. et al J. Biol. Chem 266, 16281 (1991).

Additional Info

Recognizes human p70S6K. Species Crossreactivity: mouse.

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