Ribosomal Protein S6, phosphorylated (Ser244, Ser247)

40S ribosomal protein S6 (also known as RPS6) is a ~31kD substrate of p70 S6 kinase (p70S6K) and a major component of translational machinery involved in protein synthesis, cell growth, proliferation, and metabolism. Phosphorylation of RPS6 is rapamycin and wortmannin-sensitive as its activation is mediated by mTOR and PI3K pathways. Ribosomal protein S6 undergoes phosphorylation on multiple serines in the carboxyl terminal region in the order 236?235?240?244?247, due to the positions of these amino acid residues on the a-helix. Hyperphosphorylation of ribosomal protein S6 stimulates protein synthesis that mediates\nprogression through the cell cycle.\n\nApplications: \nSuitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilution: \nWestern Blot: 1:1000 dilution. \nOptimal dilutions to be determined by the researcher.\n\nRecommended Positive Control: \nHeLa +/- TNF-a or anisomycin.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R2031-32B

Size

10 Blots

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS pH 7.3,1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Pende, M., et al. (2004). Mol. Cell. Biol. 24(8):3112-3124. 2. Mourani, P.M., et al. (2004 Circulation 109(10):1299-1306. 3. Lekmine, F., et al. (2004). Exp. Cell. Res. 295(1):173-182. 4. Tuhackova, Z., et al. (2004). Int. J. Mol. Med. 13(4):601-605. 5. Ly, C., et al. (2003) Cancer Res. 63(18):5716-5722. 6. Shah, O.J., et al. (2003). J. Biol. Chem. 278(18):16433- 16442. 7. Stewart, M.J. and G. Thomas (1994) Mitogenesis and protein synthesis: a role for ribosomal protein S6 phosphorylation? Bioessays. 16(11):809-815. Review. 8. Ferrari, S., et al. (1991) J. Biol. Chem. 266(33):22770-22775.

Additional Info

Recognizes human RPS6. Species Sequence Homology: mouse and rat RPS6: 100%. Does not cross-react with RPS6 phosphorylated on serines 235 and 236.