RICK (RIP-like-interacting CLARP Kinase, CARD3, CARD-containing Interleukin-1 beta-converting Enzyme-associated Kinase, CARD-containing IL-1 beta ICE-kinase, CARDIAK, CCK, GIG30, Receptor-interacting Protein 2, RIP2, RIP-2, Receptor-interacting Serine/thr

Apoptosis is mediated by death domain (DD) and /or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. DD-containing serine/threonine kinase RIP regulates Fas-induced apoptosis. A CARD containing serine/threonine kinase, designated RICK/RIP2/CARDIAK (for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively (1-3). RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induced apoptosis and activation of NF-kB and JUNK. RICK interacts with members of the TRAF family, CLARP, and caspase-1. Thus, RICK represents a novel kinase that regulates TNF and Fas induced apoptosis.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nK562\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R2031-59D

Size

50ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. McCarthy JV, Ni J, Dixit VM. J Biol Chem 273: 16968-16975 (1998). 2. Thome M, Hofmann K, Burns K, Martinon F, Bodmet JL, Mattman C, Tschopp J. Curr Biol 8: 885-888 (1998). 3. Inohara N, del Peso L, Koseki T, Chen S, Nunez G. J Biol Chem 273: 12296-12300 (1998).

Additional Info

Recognizes human RICK (RIP2, CARDIAK). Species Crossreactivity: mouse and rat.

Alternative Names

EC=2.7.11.1, Receptor-interacting Serine-threonine Kinase 2