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RICTOR (Rapamycin-insensitive companion of mTOR)

Cat no: 030599

RICTOR (Rapamycin-insensitive companion of mTOR)

Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals . mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GbL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GbL, Sin1 and rictor . The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro . This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes . This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR .\n\nApplications:\nWestern Bloting: 1:500-1:2000.\nImmunohistochemistry: 1:200-1:1000.\nImmunofluorescence: 1:200-1:1000.\nFlow cytometry: 1:200-1:400.\nELISA: Suggested dilution 1:10000.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

030599

Size

100ul

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, NHP

Form

Ascites fluid, 0.03% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Genes Dev. 2006 Oct 15;20(20):2820-32.\n2. Biochem Biophys Res Commun. 2008 Aug 8;372(4):578-83.

Additional Info

Human, Monkey, Mouse

Alternative Names

PIA; mAVO3; KIAA1999; MGC39830; DKFZp686B11164; RICTOR

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