Rictor (Rapamycin Insensitive Companion of mTOR, Rapamycin-insensitive Companion of mTOR, RICTR, AVO3 Homolog, hAVO3, KIAA1999, mAVO3, PIA)

Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GBL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GBL, Sin1 and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4).\n\nApplications: \nSuitable for use in Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution: \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R2031-62N

Size

400ul

Applications

IP

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 10mM sodium HEPES, pH7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG

References

(1) Sarbassov, D.D. et al. (2004) Curr. Biol. 14, 1296-1302.(2) Sarbassov, D.D. et al. (2005) Science 307, 1098-1101. (3) Hresko, R.C. and Mueckler, M. (2005) J. Biol. Chem. 280, 40406-40416.\n(4) Ali, S.M. and Sabatini, D.M. (2005) J. Biol. Chem. 280, 19445-19448.

Additional Info

Recognizes Rictor (Sepharose Bead Conjugate). Species Crossreactivity: mouse.

Alternative Names

RPTOR Independent Companion of MTOR, Complex 2, DKFZp686B11164, MGC39830, Pianissimo