RIG-1 (Retinoic Acid Inducible Gene 1 Protein, RIG-I, Probable ATP-Dependent RNA Helicase DDX58, Dead Box Protein 58, DDX58)

RIG-I is a receptor found in the cytoplasm that recognizes viral RNA from replicating viruses in infected cells. Upon activation with intracellular RNA, a cascade is triggered resulting in the activation of NF-kappa-B, and the eventual induction of antiviral cytokines such as IFN-beta and RANTES. RIG-I is essential for the production of interferons in response to RNA viruses and as such play a key role in innate immunity.\n\nApplications:\nSuitable for use in Immunohistochemistry (paraffin) and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 5ug/ml\nWestern Blot: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Human heart\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R2031-71D

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Akira S, et al (2006) Pathogen recognition and innate immunity 124:783-801. 2. Yoneyama M et al (2004) The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses. Nat. Immunol.; 5:730-737. 3. Sharma S, et al (2003) Triggering the interferon antiviral response through an IKK-related pathway Science 300:1148-51. 4. Alexopoulou L, et al (2001) Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3 Nature 413:732-8

Additional Info

Recognizes human RIG-I. Species Crossreactivity: mouse, rat.