RIG-I, ID (Probable ATP-dependent RNA Helicase DDX58, DEAD Box Protein 58, Retinoic Acid-inducible Gene 1 Protein, RIG-1, Retinoic Acid-inducible Gene I Protein, RIGI, DDX58)

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala- Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. The caspase recruitment domain (CARD)-containing intracellular sensors, RIG-I, melanoma differentiation- associated gene 5 (MDA5), and nucleotide binding and oligomerization domain (NOD), play important roles in the detection of conserved molecular structures of invading microbes. Specifically, RIG-I and MDA5 function as cytosolic receptors for viral 5'-triphosphate single- stranded RNA and double-stranded RNA (dsRNA) respectively, whereas NOD1 and NOD2 act as intracellular sensors for bacterial peptidoglycans. Upon ligand binding and activation, these intracellular pattern recognition receptors (PRRs) interact through their CARDs with downstream CARD-containing molecules to ultimately initiate a signaling cascade, resulting in the production of IFN-a/b and inflammatory cytokines to limit viral or bacterial proliferation.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:5000-1:20,000\nWestern Blot: 1:500-1:1000\nImmunohistochemistry: 1:50-1:200\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R2031-71L

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Hiscott J, et al. (2006) Trends Mol Med 12:53-56. 2. Akira S. et al. (2006) Cell 124:783-801. 3. Meylan E, et al. (2006) Nature 442:39-44. 4. Creagh EM, et al. (2006) Trends Immunol 27:352-357. 5. Hornung V, et al. (2006) Science 314:994-997. 6. Pichlmair A, et al. (2006) Science 314:997-1001. 7. Girardin SE, et al. (2003) Science 300:1584-1587. 8. Girardin SE, et al. (2003) J Biol Chem 278:8869-8872. 9. Kawai T, Akira S (2007) J Biochem 141:137-145. 10. Garcia-Sastre A, Biron CA (2006) Science 312:879-882. 11. Gack MU, et al. (2008) Proc Natl Acad Sci USA 28;105(43):16743-8.

Additional Info

Recognizes human RIG-I. Species Crossreactivity: mouse and rat.