RIG-I (RIG-1, Retinoic Acid-inducible Gene 1 Protein, DEAD-box Protein 58, Probable ATP-dependent RNA Helicase DDX58)

RIG-I and MDA5 are highly conserved helicases involved in the innate immune response to virus. RIG-I is a member of the DEAD-box RNA helicases and is activated by cytoplasmic dsRNA and 5'-ppp RNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-I recruits the CARD adaptor inducing IFN-beta (Cardif) in a CARD-CARD-dependent manner resulting in NF-kappaB and IRF3 activation.\n\nApplications:\nSuitable for use in Immunohistochemistry, Immunoprecipitation, Western Blot\n\nRecommended Dilution:\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

045257

Size

100ug

Applications

IHC, IP, WB

Hosts

Mouse

Form

Supplied as a liquid in PBS, 10% glycerol, 0.02% sodium azide.

P Type

Mab

Purity

>95% (SDS-PAGE)

Isotype

IgG1

References

Product Reference: \n?The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases: M. Rebsamen, et al.; Cell Death Differ. 15, 1804 (2008)\n?Phosphorylation-mediated negative regulation of RIG-I antiviral activity: M.U. Gack, et al.; J. Virol. 84, 3220 (2010)\n?

Additional Info

Recognizes human and mouse RIG-I.

Alternative Names

RIG-1, Retinoic Acid-inducible Gene 1 Protein, DEAD-box Protein 58, Probable ATP-dependent RNA Helicase DDX58