RING Finger Protein 25 (RNF25, E3 Ubiquitin-protein Ligase RNF25, AO7)

RING finger protein 25 (RNF25, also named AO7)contains a RING finger domain and is ubiquitously expressed in various tissues. RNF25 was initially identified in a yeast two-hybrid screen of a murine T-cell library by using UbcH5b, an E2 enzyme, as bait. RNF25 has also been shown to act as a putative E3 ligase, at least in vitro. RNF25 localizes predominantly in the nucleus and supports the transcriptional activity of NF-kB by interacting with p65 in vivo upon stimulation with TNF. Yeast two-hybrid data also suggest that RNF25 interacts with a number of other molecules which may be potential ubiquitin ligase substrates. Among these are molecules that have critical roles in signal transduction and in regulation of translation.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:10,000-1:40,000\nWestern Blot: 1:200-1:2000\nImmunoprecipitation: 1mg\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

168481

Size

100ug

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human RNF25. Species Crossreactivity: mouse.

Alternative Names

AO7 antibody, E3 ubiquitin protein ligase RNF25 antibody, EC 6.3.2.- antibody, FLJ13906 antibody, Ring finger protein 25 antibody, RING finger protein AO7 antibody, RNF 25 antibody