RIP3 (Receptor Interacting Protein 3, Receptor-interacting Protein 3, RIP-3, Receptor-interacting Serine/threonine Kinase 3, Receptor-interacting Serine/threonine-protein Kinase 3, RIP-like Protein Kinase 3, RIPK3)

RIP3 is a novel member of RIP Ser/Thr kinase family. It is a potent inducer of apoptosis and is an important component of TNFR1 signaling complex. RIP3 consists of an N-terminal RIP-like-Kinase domain and a unique, non-homologous C-terminus responsible for caspase activation and apoptosis induction. RIP3 is expressed in multiple tissues including hematopoietic cells and plays a functional role in the regulation of apoptosis and NF-kB signaling. It is recruited to the TNFR1 signaling complex in a RIP-dependent manner where it induces apoptosis by activating caspases and/or inhibiting TNFR1-induced NF-kB activation. RIP3 negatively regulates the TLR3-Trif?mediated NF-kB signaling pathway by competing with the binding of Trif to RIP1. The human RIP3 gene is localized in the chromosomal region 14q11.2.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-3ug/ml\nImmunohistochemistry (paraffin): 10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nNIH3T3 cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

R2031-74J

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Yang et.al JBC 279 (37); 38820-38829 (September 10, 2004) 2. Newton et.al Mol Cell Biol 24 (4) 1464-1469 (February 2004) 3. Yang et.al Biochem Biophy Res Comm 332 (1) 181-187 (24 June 2005) 4. Etienne M. et al. Nature Immunology. 5:503-507 (2004).

Additional Info

Recognizes human RIP3. Species Crossreactivity: chimpanzee.

Alternative Names

EC=2.7.11.1