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RIPA Lysis Buffer

Cat no: R2031-75

RIPA Lysis Buffer

RIPA lysis buffer is used to lyse cultured cells for immunoprecipitation and immunoblots. Similar in function to single detergent buffers that include NP-40, sodium chloride and Tris, RIPA is a good first choice for new antigens.\n\nRecommended Dilution:\nImmediately prior to use, add the protease and phosphatase inhibitors for the following final concentrations: 1mM PMSF, 1mM sodium orthovanadate, 1mM sodium fluoride, 1ug/ml aprotinin, 1ug/ml leupeptin, 1ug/ml pepstatin\n\nStorage and Stability: \nMay be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for at least 6 months. Store in the dark.

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SPECIFICATIONS

Catalog Number

R2031-75

Size

10ml

Applications

IP, WB

References

US Biological application references: 1. Zhang, Z.H. et al., (2007) Journal of Cell Science 120:4134-4143. 2. Song, K. et al., (2005) Molecular Endocrinology 19:504-515. 3. Due, M.R. et al. (2012) J. Neuroinflammation 9:200. 1. Antibodies: A Laboratory Manual, Ed Harlow, David Lane, Cold Spring Harbor Press, 449 (1988).

Alternative Names

150mM NaCl, 1.0% NP-40, 0.5% deoxycholate, 0.1% SDS, 50mM Tris-Cl

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