RIPK2, Recombinant, (ripk 2, RIPK-2, Active RIPK2, RIPK2, RIPK, human RIPK2, active kinase, active kinases, recombinant RIPK2, recombinant RIPK, kinase activity assay, protein kinase)

RIPK2 (RIP2; RICK) is a death domain-containing protein kinase. Inohara identified cDNAs encoding a predicted 540aa protein RICK, which contains an N-terminal serine/threonine kinase catalytic domain and a C-terminal caspase activation and recruitment domain. Inohara also demonstrated that RICK is a novel kinase that may regulate apoptosis induced by the FAS receptor pathway (1). McCarthy found that overexpression of RIP2 signaled both cell death and NF-kappa-B activation (2). Thome reported that RICK specifically interacted with the CARD of ICE/caspase-1, and this interaction correlated with the processing of pro-caspase-1 and the formation of the active caspase-1 p20 (3). Chin generated Ripk2-deficient mice and concluded that RIPK2 is implicated in the innate response to pathogens by NOD and TLR-induced cell signaling and mediates cytokine-induced Ifng production in Th1 and NK cells (4). Also Kobayashi demonstrated that RIPK2 is required for signaling through both TLR and NOD protein family members in the innate immune system as well as for appropriate TCR signaling in the adaptive immune response (5).\n\nSources:\nRecombinant corresponding to RIPK2 expressed in Sf9 insect cells.\n\nMolecular Weight:\n~59.0kD\n\nBiological Activity:\n20nmol phosphate incorporated into MBP/minute/mg protein at 30 degrees C for 15 minutes using a final concentration of 50um ATP (0.83uCi/assay).\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

167953

Size

5ug

Form

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Purity

~90% (SDS-PAGE)

References

References 1. Inohara, N.; del Peso, L.; Koseki, T.; Chen, S.; Nunez, G.: RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis. J. Biol. Chem. 273: 12296-12300, 1998. Note: Erratum: J. Biol. Chem 273: 18675 only, 1998. 2. McCarthy, J. V.; Ni, J.; Dixit, V. M.: RIP2 is a novel NF-kappa-B-activating and cell death-inducing kinase. J. Biol. Chem. 273: 16968-16975, 1998. 3. Thome, M.; Hofmann, K.; Burns, K.; Martinon, F.; Bodmer, J.-L.; Tschopp, J.: Identification of CARDIAK, a RIP-like kinase that associates with caspase-1. Curr. Biol. 8: 885-888, 1998. 4. Chin, A. I.; Dempsey, P. W.; Bruhn, K.; Miller, J. F.; Xu, Y.; Cheng, G.: Involvement of receptor- interacting protein 2 in innate and adaptive immune responses. Nature 416: 190-194, 2002. 5. Kobayashi, K.; Inohara, N.; Hernandez, L. D.; Galan, J. E.; Nunez, G.; Janeway, C. A.; Medzhitov, R.; Flavell, R. A.: RICK/Rip2/CARDIAK mediates signalling for receptors of the innate and adaptive immune systems. Nature 416: 194-199, 2002.