RNA Binding Motif Protein 8A (RNA-binding Motif Protein 8A, RBM8a, RNA Binding Protein 8A, Binder of OVCA1 1, Binder of OVCA1-1, BOV 1, BOV-1, BOV 1A, BOV-1A, BOV 1B, BOV-1B, BOV-1C, HSPC114, MDS014, Ribonucleoprotein RBM8, Ribonucleoprotein RBM8A, RNA Bi

Pre-mRNA splicing appears to play an important role in regulation of gene expression. It has been shown in a number of studies that during the splicing process, specific proteins are recruited to the mRNA and assemble into a complex of mRNA-protein near the exon-exon junction. This complex is termed mRNA-protein complex (mRNP), or the exon-exon junction complex. Several proteins were found to be present in this complex including Y14, magoh, Aly/REF, RNPS1, Upf3, and DEK.\nY14 is a 174 amino acid RNA-binding protein that contains one RNA-binding domain in the middle of the protein. Y14 binds to the spliced mRNAs in the nucleus and is exported with the mRNA to the cytoplasm. Similar to other proteins in the mRNA-protein complex, Y14 can interact with the mRNA export factor TAP and enhance the export of the mRNAs. Y14 binds to different mRNAs approx.\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: Cytoplasmic and Nuclear\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R2033-08A

Size

50ul

Applications

ELISA, IF, IP, WB

Hosts

Mouse

Reactivities

Hum, Xen/Amph

Form

0.01M PBS, pH 7.4 containing 15mM sodium azide

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2b

References

1. Kataoka, N., et al., Mol. Cell, 6, 673-682(2000).\n2. Kim, V.N., et al., EMBO J., 20, 2062-2068(2001).\n3. Kataoka, N., et al., J. Biol. Chem., 279, 7009-7013 (2004).\n4. Kim, V.N., et al., Mol. Cells, 12, 1-10 (2001).\n5. Kim, V.N., et al., Science, 293, 1832-1836 (2001).

Additional Info

Species Crossreactivity: Crossreacts with Human and Xenopus laevis.