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RNA MS2 Phage (Ribonucleic Acid)

RNA MS2 Phage (Ribonucleic Acid)

Cat no: R2033-18

Supplier: United States Biological
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Ribonucleic acids are long-chain polymers of nucleotides linked through 3',5'-phosphodiester bonds. Constituent bases are derivatives of purine and pyrimidines, the sugar is D-ribose. The RNA nucleotide sequence, which is genetic information transferred from DNA, acts as a template in sequencing amino acids in protein synthesis. MS2 RNA is a linear molecule of 3,569 nucleotides with a molecular weight of 1.2x10e6. The RNA from bacteriophage MS2 has been extensively studied as natural RNA in vivo and in vitro protein synthesis. Many of the single steps in initiation, elongation and termination of polypeptide synthesis have been worked out using MS2 RNA. The nucleotide sequence of MS2 RNA has been established facilitating structural and functional studies. MS2 RNA contains genetic information to specify three viral proteins: A-protein (structural), coat protein (structural) and replicase. It contains a high degree of secondary structure. Appearance: Colorless, clear, complete Size: 3569bp Concentration: ~0.5mg/ml RNA (A260): ~20u/ml 1unit (A260) of RNA is ~40ug Quality Control:
Catalogue number: R2033-18
Size: 5U
Form: Supplied as a liquid in 10mM Tris-HCl, pH 7.5, 1mM EDTA.
References: 1. Fiesa, W., et al., 1976, Nature, 260: 500. 2. Kozar, M., et al., 1972, Bacterial, 36: 109. 3. Capecchi, M.R., et al., 1975, Cold Spring Harbor Lab., N.Y., 279. 4. Wiltman, H., et al., 1976, Eur. J. Biochem., 61: 1. 5. Hunt, T., 1980, Trends Biochem. Sci., 5: 178. 6. Clark, B., 1980, Trends Biochem. Sci., 5: 207. 7. Caskey, C.T., 1980, Trends Biochem. Sci., 5: 234. 8. Iserentant, D., et al., 1979, J. Biol Chem., 102: 595. 9. Eckhardt, H., 1979, J. Biol Chem., 254: 11,185. 10. van Knippenberg, P.H., et al., J. Biol Chem., 254: 9,085.

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