RNA Polymerase II (MGC75453, B220, DNA-directed RNA Polymerase II Largest Subunit, hRPB220, hsRPB1, POLR2, POLR2A, POLRA, RNA Polymerase II (DNA directed) Polypeptide A 220kD, RPB1, RPB220, RPBh1, RpIILS, RPO2, RPOL2)

The carboxy-terminal repeat domain (CTD) of the largest subunit of RNA Pol II contains tandem repeats of a heptapeptide sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser which is highly conserved among eukaryotic organisms. There are two forms of RNA Pol II in vivo, designated IIO, which is extensively phosphorylated at the CTD, and IIA, which is not phosphorylated. The IIA \nform preferentially enters the pre-initiation complex (PIC), whereas IIO is found in the elongating complex. The kinase activity of TFIIH can mediate CTD phosphorylation, although other kinases, including Cdc2, Ctk1, the Srb10-Srb11 kinase-cyclin pair, and P-TEFb, have also been implicated in CTD phosphorylation. A phosphatase responsible for the dephosphorylation of the CTD has also been identified. CTD phosphatase activity is regulated by TFIIB and TFIIF. The CTD has also been implicated in pre-mRNA processing, most likely functioning as a platform for the \nrecruitment and assembly of factors involved in pre-mRNA processing. a-RNA Pol II (CTD) is a mouse monoclonal antibody (ascites) without further purification. \n

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SPECIFICATIONS

Catalog Number

R2039-20A

Size

125ul

Hosts

Mouse

Form

Liquid. Supplied as ascites. \n

P Type

Mab

Purity

Ascites

Isotype

IgG2a

References

1. Lu et al. (1991) Proc. Natl. Acad. Sci. USA 88:10004. \n2. Dahmus ME (1994) Prog. Nucleic Acid Res. Mol. Biol. 48:143. \n3. O

Additional Info

a-RNA Pol II (CTD) reacts with the CTD subunit in HeLa nuclear extract by Western \nblotting. Recommended dilution range for Western blot analysis: 1:2000 - 1:5000. \n