RNA Polymerase SP6, 20u/ul

Purified from an E.coli strain carrying a plasmid with the cloned gene encoding this enzyme. SP6 RNA Polymerase has a stringent specificity for its own promoter sequences and catalyzes the synthesis of RNA from ribonucleoside triphosphates in the presence of a DNA template.\n\nApplications:\nGeneration of strand-specific RNA sequences (see Protocol for Synthesis of Strand-specific RNA Sequences and Protocol for Synthesis of High Specific Activity Radiolabeled RNA Sequences) that may be used:\nProbes for hybridization (1) and genomic DNA sequencing (2),

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SPECIFICATIONS

Catalog Number

R2049

Size

2000u

References

1. Melton, D.A., et al., Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter, Nucleic Acids Res.,12, 7035-7056, 19842 2. 2. Church, G.M., Gilbert, W., Genomic sequencing, Proc. Natl. Acad. Sci. USA, 81, 1991-1995, 1984\n3. Peebles, C.L., et al., A self-splicing RNA excises an intron lariat, Cell, 44, 213-223, 19864. \n4. Melton, D.A., Injected antisense RNAs specifically block messenger RNA translation in vivo, Proc. Natl. Acad. Sci. USA, 82, 144-148, 19855. \n5. Krieg, P.A., Melton, D.A., Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs, Nucleic Acids Res., 12, 7057-7070, 19846.\n6. Krainer, A.R., et al., Normal and mutant human beta-globin pre-mRNAs are faithfully and efficiently spliced in vitro, Cell, 36, 993-1005, 19847\n7. Witherell, G.W., et al., Cooperative binding of R17 coat protein to RNA, Biochemistry, 29, 11051-11057, 1990.